Potion effect won't go away

I made a procedure where when you put a specific item in a GUI, it will give you potion effects when the item with the GUI is in your inventory.  when you put the item in the GUI, it works.  However, when you take it out, it keeps the potion effect on.  

here is the procedure and the GUI.  Please tell me what I did wrong and how I can fix it.  https://imgur.com/a/HhKiIds